Fungal Microscopy: Lactophenol Cotton Blue (LPCB) Stain

DG of Health Services and Programmes

Acknowledgment

The diagnostic laboratories services at the Directorate General of Specialized Medical Care (DGSMC) at Ministry of Health (MOH) would like to thank and appreciate the great effort of the Microbiology documents development team. Participated and contributed personnel are:

Member name	Institution	Designation
Dr.Mahmoud Al Subhi	Rustaq Hospital	Team Leader Specialist microbiologist pathologist
Ms. Zainab Al Hadhrami	Directorate General of Specialized Medical Care	Team Coordinator Head of Quality unit in Diagnostic laboratories services
Ms. Saleh Al Shukairi	Ibra Hospital	Senior technologist specialist A
Dr. Hanaa Al Auraimi	Royal Police of Oman Hospital	Specialist microbiologist pathologist
Dr. Nawal AL Kindi	Khoula Hospital	Specialist microbiologist pathologist
Dr. Al Warith Al Kharosi	Nizwa Hospital	Specialist microbiologist pathologist
Dr. Abdulrahman Al Mahrouqi	Ibri Hospital	Specialist microbiologist pathologist
Dr. Nada Al Tamimi	Al Massara Hospital	Specialist microbiologist pathologist
Dr. Wafaa Al Tamtami	Armed Forces Hospital	Specialist microbiologist pathologist

Acronyms:

LPCB	Lactophenol cotton blue stain
H&S	Health and Safety
ID	Identification
IQC	Internal Quality Control
CPHL	Central Public Health Laboratory

1 - Purpose

This document describes the procedure for fungal microscopy using Lactophenol cotton blue (LPCB).